The patient cohort that was studied using protein arrays included individuals with APS1 from Sweden, Finland and Norway and healthy blood donors from Sweden, as described previously Landegren et al. However, as pointed out in the formal response, Landegren failed to sufficiently quantify neutralization. Langan San Francisco langan. We followed the analytical approach used as closely as possible from the information available in the published article Meyer et al. Gannett Fleming Los Angeles gannettfleming. Hobart Yard Pavement Improvement Commerce 3.
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A We used our previously published proteome array dataset for 51 APS1 patients and 21 healthy controls to reevaluate the autoantigen spectrum in APS1.
The study was conducted in accordance with the Helsinki declaration. Cowi North America Inc. Cite this article as: eLife ;8:e doi: LPA Inc.
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Two of those observations have been challenged. This would be especially important for determining the relevance if any of the type of rare autoantibody signals that are observed both among patients and healthy controls. The example thereby revealed that even extremely elevated values in the control group could be missed in the analysis.
We thank Cindy Wong for critical review of the manuscript. Lionakis Sacramento lionakis. Henmi, CEO.
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We attempted to validate this finding in a larger cohort of APS1 subjects with type 1 diabetes and could find no evidence to support this proposed association.
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They can conclude that the APECED patients who develop diabetes in their cohort have type 1 IFN neutralizing autoantibodies, but they cannot comment on whether the titer is the same or different to the APECED patients without diabetes since they have reached assay saturation in both groups.
Steve Ro, CEO. The positive and negative controls in the experiments also verified a reliable detection of neutralizing activity. Protein array technology provides the opportunity for mapping autoantibody targets on a broad scale. Figure 2.
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|Pleasanton Dahlingroup. Here we used other approaches to also address rare autoantigens in APS1. Landegren, N. To address this question, we used proteome array data for 51 APS1 patients and 21 healthy controls Landegren et al.
In it was reported that: i the spectrum of autoantibodies in patients with AIRE -deficiency is much broader than previously appreciated; ii neutralizing autoantibodies to type I interferons IFNs could provide protection against type 1 diabetes in these patients Meyer et al.